| Type: | IVD Reagent |
|---|---|
| Material: | Plastic |
| Quality Guarante: | 2 Years |
| Sensitivity: | 92.15%-99.05% |
| Specificity: | 97.47%-99.61% |
| Accuracy: | 97.19%-99.33% |
| Samples: |
|---|
| Customization: |
|---|
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[Product name]
Interleukin-6(IL-6)Test Kit (Fluorescence immunochromatography)
[Packaging specifications]
10 tests/box, 20 tests/box, 25 tests/box, 30 tests/box, 50 tests/box, 100 tests/box.
[Indicated use]
The kit is used for quantitative determination of interleukin-6 (IL-6) in human serum, plasma or whole blood samples in vitro.
Interleukin-6 is mainly produced by Th2 cells and secreted by T lymphocytes, B lymphocytes, fibroblasts, endothelial cells, keratinocytes, hepatocytes and bone marrow cells. Its relative molecular weight is 26kDa. It is a glycoprotein composed of 212 amino acid residues. Interleukin-6 is the first biomarker to appear after inflammatory reaction. In different inflammatory diseases, the content of interleukin-6 is significantly different. The increased level of interleukin-6 caused by bacterial infection is significantly higher than that caused by non-bacterial infection, and the level of interleukin-6 is directly proportional to the severity of inflammation and infection. Interleukin-6 is also a sensitive early warning index of sepsis, and has a good correlation with the severity and prognosis of sepsis. As an inflammatory factor, the level of interleukin-6 is also closely related to cardiovascular and cerebrovascular diseases such as atherosclerosis and acute myocardial infarction (AMI).
[Test principle]
This test kit uses fluorescence immunochromatography technology to quantitatively detrmine the content of interleukin-6 (IL-6) in serum, plasma or whole blood samples. After the sample is added to the test card, IL-6 in the sample reacts with fluorescent labeled IL-6 monoclonal antibody 1 to form a complex, which continues to chromatography forward along the nitrocellulose membrane to the test line, and forms a complex with coated IL-6 monoclonal antibody 2 to bind on the test line. Under the action of excitation light source, the content of interleukin-6 (IL-6) in the sample is positively correlated with the fluorescent antibody signal, and the content of interleukin-6 (IL-6) in the sample is calculated by fluorescence immunoanalyzer.
Principle of the supporting instrument: the measuring system of the instrument scans the binding area between the marker and the analyte on the test card after the reaction, and obtains the optical signal. Then the optical signal is measured and analyzed to quantitatively obtain the concentration of the analyte.
[Main components]
|
NO. |
Name |
Components |
|
1 |
interleukin-6 (IL-6) Test Kit (fluorescence immunochromatography) |
Each pouch contains a test card and a desiccant; the test card is composed of a shell and a test strip, the test strip is composed of sample pad, fluorescent pad (fixed with fluorescent labeled IL-6 monoclonal antibody 1), nitrocellulose membrane (coated with IL-6 monoclonal antibody 2 and goat anti-mouse IgG), filter paper and PVC plastic plate |
|
2 |
Whole blood diluent |
1 bottle of diluent for 10 tests/box, 20tests/box, 25 tests/box, 30 tests/box; 2 bottles of diluent for 50 tests/box; 2 bottles of diluent for 100 test/box; 3.0mL/bottle |
|
3 |
ID card |
1pc/box |
[Applicable instrument]
Fluorescence immunoanalyzer
[Storage conditions and validity period]
The test kit should be stored in a sealed aluminum foil bag at 4ºC~30ºC, and the validity period is 18 months. After the foil bag is opened, the validity period is 1 hour.
[Test method]
Please read the instructions of the test kit and the manual of the fluorescence immunoanalyzer carefully before use.
a) Bring the test kit and sample to be tested to room temperature.
b) Make sure the ID card matches the batch number of the kit, and insert the ID card into the card reading area of the instrument to read the information.
c) Open the inner package of the test card, take out the test card; draw 75μL of serum plasma sample, drop vertically to the test card sampling place, and start timing; draw 75μL whole blood, vertically drop it to the test card sampling place, and immediately add 1 drop whole blood diluent at the sampling place and start timing.
d) After adding the sample, click "Start Test" on the screen of the fluorescence immunoanalyzer, and the test card will react at room temperature for 15 minutes; insert the test card into the test card slot of the fluorescence immunoanalyzer, and the instrument will automatically test the test card; The test results can be seen on the display screen of the immunoassay analyzer. Click "Print" on the screen to print the results.
[Positive judgment value or reference interval]
Normal reference value:<7pg/mL.
Due to differences in geography, race, gender and age, it is recommended that each laboratory establish its own positive judgment value or reference interval.
[Interpretation of test results] (For reference only, not used as clinical diagnostic criteria, test results need to be combined with other clinical and laboratory data for clinical diagnosis)
|
Content of IL-6(pg/mL) |
Clinical application suggestions |
|
<7 |
Normal value |
|
7-150 |
Indicates mild inflammation or mild infection. |
|
150-250 |
Suggests general bacterial infection or systemic inflammatory reaction. |
|
>250 |
Suggests may be sepsis. |
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Audited Supplier 
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